Key features and details
- Rabbit polyclonal to PKA R2/PKR2 (phospho S96)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKA R2/PKR2 (phospho S96) antibody
See all PKA R2/PKR2 primary antibodies
DescriptionRabbit polyclonal to PKA R2/PKR2 (phospho S96)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide within Human PKA R2/PKR2 (N terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P13861
- WB: HEK-293T whole cell extracts.
This product was previously labelled as PKA R2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsPhosphorylated by the activated catalytic chain.
Cellular localizationCytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
- Information by UniProt
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
All lanes : Anti-PKA R2/PKR2 (phospho S96) antibody (ab226754) at 1/3000 dilution
Lane 1 : Untreated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : CIP treated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226754 has not yet been referenced specifically in any publications.