Recombinant Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E151] to PKA R2/PKR2 (phospho S99) - BSA and Azide free
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
Related conjugates and formulations
Overview
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Product name
Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free
See all PKA R2/PKR2 primary antibodies -
Description
Rabbit monoclonal [E151] to PKA R2/PKR2 (phospho S99) - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody only detects PKA R2/PKR2 phosphorylated on Serine 99. Phosphoserine 99 in Human corresponds to phosphoserine 96 in mouse.
The mouse and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse and pig.
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Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells. IHC-P: Human lymph node metastatic tissue. Human kidney without Lambda Protein Phosphatase treatment (image A) and Rat kidney without Lambda Protein Phosphatase treatment
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General notes
ab238951 is the carrier-free version of ab32390.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E151 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse and pig. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse and pig. |
Target
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Function
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. -
Tissue specificity
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. -
Sequence similarities
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains. -
Post-translational
modificationsPhosphorylated by the activated catalytic chain. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5576 Human
- Entrez Gene: 19087 Mouse
- Entrez Gene: 29699 Rat
- Omim: 176910 Human
- SwissProt: P13861 Human
- SwissProt: P12367 Mouse
- SwissProt: P12368 Rat
- Unigene: 631923 Human
see all -
Alternative names
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney without Lambda Protein Phosphatase treatment (image A) and treated with Lambda Protein Phosphatase (image B). tissue sections labeling PKA R2/PKR2 with purified ab32390 at 1:500 dilution (0.75 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney without Lambda Protein Phosphatase treatment (image A) and treated with Lambda Protein Phosphatase (image B). tissue sections labeling PKA R2/PKR2 with purified ab32390 at 1:500 dilution (0.75 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab32390, at a 1/250 dilution, staining (A) untreated (B) Phosphatase treated lymph node metastatic by immunohistochemistry, Paraffin embedded human tissue
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32390).
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Cell line: HeLa (human cervix adenocarcinoma)
Target AbID: Ab32390 anti-PKA R2/PKR2 (phospho S99)
Target Secondary: ab150077 AlexaFluor®488 goat anti-rabbit
Counterstain AbID: ab7291 anti-Tubulin (mouse mAb)
Target Secondary: ab150120 AlexaFluor®594 goat anti-mouse
Fixative: 4% PFA
Permeabilization: 0.1% Triton-X
Nuclear counter stain: DAPI
Other comments: Confocal image showed increased cytoplasmic staining after CA (100ng/ml, 10min) treatment on Hela cells. The LP treatment decreased the increased cytoplasmic staining caused by CA. Ab32514 was used as a Pan control for ab32390. The results showed cytoplasmic staining on untreated, CA and CA+LP treated Hela cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32390).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab238951 has not yet been referenced specifically in any publications.