Validated using a knockout cell line

Recombinant Anti-PKC alpha antibody [Y124] (ab32376)

Knockout Tested Rabbit recombinant monoclonal PKC alpha antibody [Y124]. Validated in WB, IP, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human, Pig, Goldfish. Cited in 37 publication(s).

Publishing research using ab32376? Please let us know so that we can cite the reference in this datasheet.

ab32376 has been referenced in 44 publications.

  • Miranda-Silva D  et al. Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload. Sci Rep 9:2956 (2019). PubMed: 30814653
  • Zou T  et al. Organoid-derived C-Kit+/SSEA4- human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents. Nat Commun 10:1205 (2019). PubMed: 30872578
  • Wang Y  et al. Mechanism of Regulation of Big-Conductance Ca2+-Activated K+ Channels by mTOR Complex 2 in Podocytes. Front Physiol 10:167 (2019). PubMed: 30873046
  • Ji Y  et al. Astragalosides increase the cardiac diastolic function and regulate the "Calcium sensing receptor-protein kinase C-protein phosphatase 1" pathway in rats with heart failure. Biomed Pharmacother 103:838-843 (2018). WB . PubMed: 29710499
  • Dandekar MP  et al. Intravenous infusion of xenon-containing liposomes generates rapid antidepressant-like effects. Prog Neuropsychopharmacol Biol Psychiatry 86:140-149 (2018). WB . PubMed: 29559371
  • Li G  et al. Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells Int 2018:4968658 (2018). PubMed: 30008752
  • Li X  et al. Activation of protein kinase C-a/heme oxygenase-1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide-activated NR8383 cells. Exp Ther Med 16:1529-1537 (2018). PubMed: 30112072
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
  • Zaidman NA  et al. Large-conductance Ca(2+) -activated K(+) channel activation by apical P2Y receptor agonists requires hydrocortisone in differentiated airway epithelium. J Physiol 595:4631-4645 (2017). PubMed: 28481415
  • Ono A  et al. Feedback activation of AMPK-mediated autophagy acceleration is a key resistance mechanism against SCD1 inhibitor-induced cell growth inhibition. PLoS One 12:e0181243 (2017). PubMed: 28704514
  • Zhang HM  et al. Moderate lifelong overexpression of tuberous sclerosis complex 1 (TSC1) improves health and survival in mice. Sci Rep 7:834 (2017). WB . PubMed: 28400571
  • Guo Y  et al. Clinical significance of the correlation between PLCE 1 and PRKCA in esophageal inflammation and esophageal carcinoma. Oncotarget 8:33285-33299 (2017). PubMed: 28402280
  • Haemmerle M  et al. Platelets reduce anoikis and promote metastasis by activating YAP1 signaling. Nat Commun 8:310 (2017). PubMed: 28827520
  • Hickey DG  et al. Tropism of engineered and evolved recombinant AAV serotypes in the rd1 mouse and ex vivo primate retina. Gene Ther 24:787-800 (2017). Mouse . PubMed: 28872643
  • Kelsey JS  et al. Biological activity of the bryostatin analog Merle 23 on mouse epidermal cells and mouse skin. Mol Carcinog 55:2183-2195 (2016). PubMed: 26859836
  • Sanges D  et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. J Clin Invest 126:3104-3116 (2016). IHC-P ; Mouse . PubMed: 27427986
  • Termini CM  et al. Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCa in Acute Myeloid Leukemia. Sci Rep 6:29859 (2016). IHC-P ; Human . PubMed: 27417454
  • Kötter S  et al. Titin-Based Cardiac Myocyte Stiffening Contributes to Early Adaptive Ventricular Remodeling After Myocardial Infarction. Circ Res 119:1017-1029 (2016). PubMed: 27650557
  • Ouk K  et al. Attenuated pupillary light responses and downregulation of opsin expression parallel decline in circadian disruption in two different mouse models of Huntington's disease. Hum Mol Genet 25:N/A (2016). PubMed: 28031289
  • Wang XH  et al. Cannabinoid CB1 receptor signaling dichotomously modulates inhibitory and excitatory synaptic transmission in rat inner retina. Brain Struct Funct 221:301-16 (2016). PubMed: 25273281
  • Lum MA  et al. PKCa is Resistant to Long-Term Desensitization/Downregulation by Prolonged Diacylglycerol Stimulation. J Biol Chem N/A:N/A (2016). WB, IHC, ICC/IF ; Rat, Human . PubMed: 26769967
  • Yang GY  et al. Influence of orally fed a select mixture of Bacillus probiotics on intestinal T-cell migration in weaned MUC4 resistant pigs following Escherichia coli challenge. Vet Res 47:71 (2016). WB . PubMed: 27424033
  • Graffe M  et al. A marginal band of microtubules transports and organizes mitochondria in retinal bipolar synaptic terminals. J Gen Physiol 146:109-17 (2015). ICC/IF ; Goldfish . PubMed: 26123197
  • Liu Q  et al. Adapting a drug screening platform to discover associations of molecular targeted radiosensitizers with genomic biomarkers. Mol Cancer Res 13:713-20 (2015). WB ; Human . PubMed: 25667133
  • Heiserman JP  et al. TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes. Cell Stress Chaperones 20:527-35 (2015). WB ; Mouse . PubMed: 25716072
  • Di Nisio C  et al. A dual role for ß1 integrin in an in vitro Streptococcus mitis/human gingival fibroblasts co-culture model in response to TEGDMA. Int Endod J 48:839-49 (2015). PubMed: 25231818
  • Zhang W  et al. A Selected Lactobacillus rhamnosus Strain Promotes EGFR-Independent Akt Activation in an Enterotoxigenic Escherichia coli K88-Infected IPEC-J2 Cell Model. PLoS One 10:e0125717 (2015). WB ; Pig . PubMed: 25915861
  • Bailey TA  et al. A kinase inhibitor screen reveals protein kinase C-dependent endocytic recycling of ErbB2 in breast cancer cells. J Biol Chem 289:30443-58 (2014). WB, ICC/IF ; Human . PubMed: 25225290
  • Wang M  et al. EGFR-mediated chromatin condensation protects KRAS-mutant cancer cells against ionizing radiation. Cancer Res 74:2825-34 (2014). WB ; Human . PubMed: 24648348
  • Pysz MA  et al. Differential regulation of cyclin D1 expression by protein kinase C a and ? signaling in intestinal epithelial cells. J Biol Chem 289:22268-83 (2014). WB ; Rat . PubMed: 24914206
  • El Hajj Chehadeh S  et al. Early methyl donor deficiency alters cAMP signaling pathway and neurosteroidogenesis in the cerebellum of female rat pups. Am J Physiol Endocrinol Metab 307:E1009-19 (2014). PubMed: 25294213
  • Zhang Y  et al. GRK5 dysfunction accelerates tau hyperphosphorylation in APP (swe) mice through impaired cholinergic activity. Neuroreport 25:542-7 (2014). PubMed: 24598771
  • Gustafson-Wagner E & Stipp CS The CD9/CD81 tetraspanin complex and tetraspanin CD151 regulate a3ß1 integrin-dependent tumor cell behaviors by overlapping but distinct mechanisms. PLoS One 8:e61834 (2013). WB ; Human . PubMed: 23613949
  • Xu R  et al. Oxidative stress mediates the disruption of airway epithelial tight junctions through a TRPM2-PLC?1-PKCa signaling pathway. Int J Mol Sci 14:9475-86 (2013). WB ; Human . PubMed: 23629676
  • Hill KS  et al. Protein kinase Ca suppresses Kras-mediated lung tumor formation through activation of a p38 MAPK-TGFß signaling axis. Oncogene N/A:N/A (2013). IHC-P ; Mouse . PubMed: 23604119
  • Lum MA  et al. Agonist-induced down-regulation of endogenous protein kinase c a through an endolysosomal mechanism. J Biol Chem 288:13093-109 (2013). WB, ICC/IF ; Rat, Human . PubMed: 23508961
  • Chen JQ  et al. Absolute quantitation of endogenous proteins with precision and accuracy using a capillary Western system. Anal Biochem 442:97-103 (2013). WB ; Human . PubMed: 23896461
  • Lee E  et al. Assessment of 180° rotation of the choroid as a novel surgical treatment for age-related macular degeneration. Invest Ophthalmol Vis Sci 53:2523-32 (2012). PubMed: 22427591
  • Hao F  et al. Protein kinase Ca signaling regulates inhibitor of DNA binding 1 in the intestinal epithelium. J Biol Chem 286:18104-17 (2011). IHC-P ; Mouse . PubMed: 21454537
  • Fioravante D  et al. Calcium-dependent isoforms of protein kinase C mediate posttetanic potentiation at the calyx of Held. Neuron 70:1005-19 (2011). ICC/IF ; Mouse . PubMed: 21658591
  • Tang J  et al. Activated PKC{delta} and PKC{epsilon} inhibit epithelial chloride secretion response to cAMP via inducing internalization of the Na+-K+-2Cl- cotransporter NKCC1. J Biol Chem : (2010). WB, ICC/IF ; Human . PubMed: 20732874
  • Lafferty-Whyte K  et al. A gene expression signature classifying telomerase and ALT immortalization reveals an hTERT regulatory network and suggests a mesenchymal stem cell origin for ALT. Oncogene 28:3765-74 (2009). WB ; Human . PubMed: 19684619
  • Pysz MA  et al. PKCalpha tumor suppression in the intestine is associated with transcriptional and translational inhibition of cyclin D1. Exp Cell Res 315:1415-28 (2009). ICC/IF ; Human . PubMed: 19232344
  • Neves JS  et al. Eosinophil granules function extracellularly as receptor-mediated secretory organelles. Proc Natl Acad Sci U S A 105:18478-83 (2008). WB ; Human . PubMed: 19017810

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