Product nameAnti-PKC alpha antibody [Y124] (Phycoerythrin)
See all PKC alpha primary antibodies
DescriptionRabbit monoclonal [Y124] to PKC alpha (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Flow Cyt: HeLa cells ICC/IF: HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-PKC alpha antibody [Y124] (Alexa Fluor® 488) (ab204633)
- Anti-PKC alpha antibody [Y124] (Alexa Fluor® 647) (ab204634)
- Anti-PKC alpha antibody [Y124] (HRP) (ab205422)
- Anti-PKC alpha antibody [Y124] (Alexa Fluor® 594) (ab206344)
- Anti-PKC alpha antibody [Y124] (Alexa Fluor® 405) (ab206345)
- Anti-PKC alpha antibody [Y124] (Alexa Fluor® 568) (ab208827)
- Anti-PKC alpha antibody [Y124] - BSA and Azide free (ab221611)
- Anti-PKC alpha antibody [Y124] (ab32376)
Our Abpromise guarantee covers the use of ab208749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus.
- Information by UniProt
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
Overlay histogram showing HeLa cells stained with ab208739 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208749, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.
ab208749 staining PKC alpha in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208749 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
ab208749 has not yet been referenced specifically in any publications.