Product nameAnti-PKC alpha (phospho T497) antibody
See all PKC alpha primary antibodies
DescriptionRabbit polyclonal to PKC alpha (phospho T497)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Rhesus monkey
Synthetic peptide within Human PKC alpha (C terminal) (phospho T497). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P17252
- WB: Jurkat whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 76 kDa.|
FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus.
- Information by UniProt
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
All lanes : Anti-PKC alpha (phospho T497) antibody (ab226785) at 1/1000 dilution
Lane 1 : Untreated Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract treated with calf-intestinal alkaline phosphatase
Lysates/proteins at 30 µg per lane.
Predicted band size: 76 kDa
7.5% SDS-PAGE gel.
ab226785 has not yet been referenced specifically in any publications.