Key features and details
- Rabbit polyclonal to PKC alpha (phospho T497)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKC alpha (phospho T497) antibody
See all PKC alpha primary antibodies
DescriptionRabbit polyclonal to PKC alpha (phospho T497)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Rhesus monkey
Synthetic peptide within Human PKC alpha (C terminal) (phospho T497). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P17252
- WB: Jurkat whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 76 kDa.|
FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus.
- Information by UniProt
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
All lanes : Anti-PKC alpha (phospho T497) antibody (ab226785) at 1/1000 dilution
Lane 1 : Untreated Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract treated with calf-intestinal alkaline phosphatase
Lysates/proteins at 30 µg per lane.
Predicted band size: 76 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226785 has not yet been referenced specifically in any publications.