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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PKC alpha (phospho T497). The exact sequence is proprietary.
(Peptide available as
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab76016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||1/50000 - 1/100000. Predicted molecular weight: 77 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Blocking and diluting buffer - 5% NFDM/TBST
Blocking/Diluting Buffer - 5% NFDM/TBST
LP is Lambda Phosphatase. It is not an inducer of phosphorylation but is essential to treat the membrane (not the cells).
Dot blot analysis of PKC alpha (pT497) peptide (Lane 1) and PKC alpha non-phospho peptide (Lane 2) labelling PKC alpha (phospho T497) with ab76016 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"