Product nameAnti-PKC antibody [MC5]
See all PKC primary antibodies
DescriptionMouse monoclonal [MC5] to PKC
SpecificityThis antibody reacts with PKC alpha, crossreactivity to PKC beta isoformes can be observed.
Tested applicationsSuitable for: ICC/IF, ELISA, IP, RIA, WB, Flow Cyt, IHC-FoFr, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Purified bovine brain protein kinase C
- WB: Calpain treated PKC. Rat adipocyte homogenate. 3T3-L1 homogenate. Purified microsomes from 3T3-L1 cells. Flow Cytometry: Jurkat cells. ICC/IF: Vero and HEK-293T cells. IHC-Fr: mouse spleen and duodenum tissue. IHC-P: Human skin papilloma tissue.
It should be possible to selectively block degradation of PKC without affecting the membrane associated activation. This will allow an assessment of the role of proteolysis in the activation of the protein kinase pathway.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein A purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab31 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18368118|
|IHC-P||Use at an assay dependent concentration. PubMed: 17266784|
FunctionCalcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Cell membrane. Mitochondrion membrane. Nucleus.
- Information by UniProt
- KPCA_HUMAN antibody
- PKC alpha antibody
- PKC beta antibody
All lanes : Anti-PKC antibody [MC5] (ab31)
Lane 1 : Calpain treated PKC
Lane 2 : Homogenate from rat adipocytes
Lane 3 : Homogenate from 3T3-L1 cells
Lane 4 : Purified microsomes from 3T3-L1 cells
Review by Mikael Rutberg submitted 2 September 2004.
ab31 staining PKC in human skin papilloma tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. A biotin-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab31 staining PKC in mouse spleen and duodenum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI.
Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab31 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) ab150113 at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in HL-60 (Human promyelocytic leukemia cell line) cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
Vero cells with ab31 at a 1/50 dilution.
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells with ab31 at 1/50 dilution.
This product has been referenced in:
- Chen J et al. Roles and mechanisms of TRPC3 and the PLC?/PKC/CPI-17 signaling pathway in regulating parturition. Mol Med Rep 17:898-910 (2018). Read more (PubMed: 29115500) »
- Li Y & Luan C PLCE1 Promotes the Invasion and Migration of Esophageal Cancer Cells by Up-Regulating the PKCa/NF-?B Pathway. Yonsei Med J 59:1159-1165 (2018). Read more (PubMed: 30450849) »