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I am interested in finding a specific antibody for activated PKC beta and I'm not sure which one would be better, ab5785 or ab5817. I will be using the antibody for Western Blot analysis and also treating with LY333,351 to inhibit PKC beta I and II. My lab is concerned with PKC activation via DAG in hyperglycemia/diabetes. If you have any further information about these antibodies I would appreciate your assistance. Thank You.
Asked on Nov 15 2005
Thank you for your enquiry. Are you interested in detecting PKC beta 1 or 2? Ab5785 detects PKC beta 2 phosphorylated at T641, where as ab5817 detects PKC beta 1 and 2 phosphorylated at T500. For both of these antibodies there are Western blot images on the online datasheets, and we guarantee that the antibodies will work in Western blotting with human samples. I hope this helps. If you have any additional questions, please contact us again.
Answered on Nov 16 2005