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what are the typical conditions for PMA(TPA) stimulation of PKC ? I have found all range of time and concentrations. For instance, what are the PMA treatment conditions that have been used in the blot you showed for anti-PKC beta1-beta2 T500 (ab5817-50 ?
Asked on Jun 16 2004
Thank you for your enquiry. Useful working concentration range of PMA is 1–100 nM. You may need to consider testing the half-maximal effective dose of PMA in your experimental system. Please take a look at the following website, you may find some useful information on PMA and effective concentration: http://www.promega.com/cnotes/cn001/cn001_10.pdf
Answered on Jun 17 2004