Question (8510) | Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody (ab5817)

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Question

what are the typical conditions for PMA(TPA) stimulation of PKC ? I have found all range of time and concentrations. For instance, what are the PMA treatment conditions that have been used in the blot you showed for anti-PKC beta1-beta2 T500 (ab5817-50 ?

Answer

Thank you for your enquiry. Useful working concentration range of PMA is 1–100 nM. You may need to consider testing the half-maximal effective dose of PMA in your experimental system. Please take a look at the following website, you may find some useful information on PMA and effective concentration: http://www.promega.com/cnotes/cn001/cn001_10.pdf

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