Product nameAnti-PKC beta 1 antibody
See all PKC beta 1 primary antibodies
DescriptionRabbit polyclonal to PKC beta 1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Cow, Rhesus monkey
Recombinant fragment within Human PKC beta 1 (internal sequence). The exact sequence is proprietary.
Database link: P05771
- WB: SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-P: Rat brain tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 76 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
Cellular localizationCytoplasm. Nucleus. Membrane.
- Information by UniProt
- KPCB_HUMAN antibody
- PKC beta antibody
- PKC-B antibody
All lanes : Anti-PKC beta 1 antibody (ab227490) at 1/1000 dilution
Lane 1 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 2 : IMR32 whole cell extract
Lane 3 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 76 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded rat brain tissue stained for PKC beta 1 using ab227490 at 1/500 dilution in immunohistochemical analysis.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PKC beta 1 (green) using ab227490 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
This product has been referenced in:
- Ge J et al. S-adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cß/C-kinase-activated protein phosphatase-1 inhibitor of 17 kDa signaling pathway. Exp Ther Med 16:103-112 (2018). Read more (PubMed: 29896230) »