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    pkc-beta-1-antibody-ab262849.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Anti-PKC beta 1 antibody (ab262849)

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Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 1 antibody (ab262849)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 1 antibody (ab262849)

Key features and details

  • Rabbit polyclonal to PKC beta 1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human PKC beta 1 protein (ab60840)

View more associated products

Overview

  • Product name

    Anti-PKC beta 1 antibody
    See all PKC beta 1 primary antibodies
  • Description

    Rabbit polyclonal to PKC beta 1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human PKC beta 1 aa 600 to the C-terminus.
    Database link: P05771

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human pancreas. ICC/IF: RT4 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant human PKC beta 1 protein (ab60840)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab262849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localization

    Cytoplasm. Nucleus. Membrane.
  • Target information above from: UniProt accession P05771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5579 Human
    • Omim: 176970 Human
    • SwissProt: P05771 Human
    • Unigene: 460355 Human
    • Alternative names

      • KPCB_HUMAN antibody
      • PKC beta antibody
      • PKC-B antibody
      • PKC-beta antibody
      • PKCB antibody
      • Prkcb antibody
      • PRKCB I antibody
      • PRKCB1 antibody
      • PRKCB2 antibody
      • Protein kinase C beta antibody
      • Protein kinase C beta type antibody
      • protein kinase C, beta 1 polypeptide antibody
      • protein kinase C, beta-1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 1 antibody (ab262849)
      Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 1 antibody (ab262849)
      PFA-fixed, Triton X-100 permeabilized human RT4 cells stained for PKC beta 1 (green) using ab262849 at 4 µg/ml in ICC/IF.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 1 antibody (ab262849)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 1 antibody (ab262849)

      Paraffin-embedded Human pancreas tissue stained for PKC beta 1 using ab262849 at 1/20 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab262849? Please let us know so that we can cite the reference in this datasheet.

    ab262849 has not yet been referenced specifically in any publications.

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