Anti-PKC beta 1 + PKC beta 2 (phospho T641) antibody (ab194749)
Key features and details
- Rabbit polyclonal to PKC beta 1 + PKC beta 2 (phospho T641)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC beta 1 + PKC beta 2 (phospho T641) antibody
See all PKC beta 1 + PKC beta 2 primary antibodies -
Description
Rabbit polyclonal to PKC beta 1 + PKC beta 2 (phospho T641) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PKC beta 1 + PKC beta 2 (phospho T641). Residues surrounding threonine 641.
Database link: P05771 -
Positive control
- Extracts from jurkat cells treated with PMA, Human lung carcinoma tissue and MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/200.
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 77 kDa.
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Notes |
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ICC/IF
1/100 - 1/200. |
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 77 kDa. |
Target
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Function
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. -
Cellular localization
Cytoplasm. Nucleus. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5579 Human
- Omim: 176970 Human
- SwissProt: P05771 Human
- Unigene: 460355 Human
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Alternative names
- KPCB_HUMAN antibody
- PKC beta antibody
- PKC-B antibody
see all
Images
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All lanes : Anti-PKC beta 1 + PKC beta 2 (phospho T641) antibody (ab194749) at 1/500 dilution
Lane 1 : Extract from jurkat cells
Lane 2 : Extract from jurkat cells treated with PMA
Predicted band size: 77 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling PKC beta 1 + PKC beta 2 (phospho T641) using ab194749 at a 1/50 dilution.
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Immunofluorescence analysis of methanol-fixed MCF7 cells labeling PKC beta 1 + PKC beta 2 (phospho T641) using ab194749 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab194749 has been referenced in 3 publications.
- Yang L et al. CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation. Cell Death Differ 28:2616-2633 (2021). PubMed: 33879857
- Guo Y et al. D-4F Ameliorates Contrast Media-Induced Oxidative Injuries in Endothelial Cells via the AMPK/PKC Pathway. Front Pharmacol 11:556074 (2020). PubMed: 33658920
- Su H et al. In utero hypoxia altered Ang II-induced contraction via PKCß in fetal cerebral arteries. J Endocrinol 244:213-222 (2020). PubMed: 31645018