Product nameAnti-PKC beta 1 + PKC beta 2 (phospho T641) antibody
See all PKC beta 1 + PKC beta 2 primary antibodies
DescriptionRabbit polyclonal to PKC beta 1 + PKC beta 2 (phospho T641)
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PKC beta 1 + PKC beta 2 (phospho T641). Residues surrounding threonine 641.
Database link: P05771
- Extracts from jurkat cells treated with PMA, Human lung carcinoma tissue and MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194749 in the following tested applications.
|ICC/IF||1/100 - 1/200.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 77 kDa.|
FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
Cellular localizationCytoplasm. Nucleus. Membrane.
- Information by UniProt
- KPCB_HUMAN antibody
- PKC beta antibody
- PKC-B antibody
All lanes : Anti-PKC beta 1 + PKC beta 2 (phospho T641) antibody (ab194749) at 1/500 dilution
Lane 1 : Extract from jurkat cells
Lane 2 : Extract from jurkat cells treated with PMA
Predicted band size: 77 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling PKC beta 1 + PKC beta 2 (phospho T641) using ab194749 at a 1/50 dilution.
Immunofluorescence analysis of methanol-fixed MCF7 cells labeling PKC beta 1 + PKC beta 2 (phospho T641) using ab194749 at a 1/100 dilution.
ab194749 has not yet been referenced specifically in any publications.