Overview

  • Product name
  • Description
    Rabbit polyclonal to PKC beta 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human, Mammals
  • Immunogen

    Synthetic peptide:

    SFVNSEFLKPEVKS

    , corresponding to amino acids 660-673 of Rat PKC beta 2.

  • Positive control
    • Brain and spleen.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC
IHC-Fr
WB
  • Application notes
    ELISA: assay dependent.
    Immunocytochemistry (frozen): 1/250 (using alkaline phosphatase or horseradish peroxidase).
    Western blot (enhanced chemiluminescence): 1/250 (using alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 80 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
  • Target

    • Function
      Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
    • Cellular localization
      Cytoplasm. Nucleus. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCB_HUMAN antibody
      • PKC Beta antibody
      • PKC-B antibody
      • PKC-beta antibody
      • PKCB antibody
      • Prkcb antibody
      • PRKCB II antibody
      • PRKCB2 antibody
      • Protein kinase C beta antibody
      • Protein kinase C beta type antibody
      see all

    References

    ab4138 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for getting back to me. Thank you also for trying my suggestions. I am sorry that they did not lead to any improvements to the results that you have been obtaining. As mentioned previously unfortunately we cannot guarantee this antibody to work in mouse as it is yet to be tested. I can only suggest that you try applying this antibody to a positive control tissue such as rat brain. I am sorry that I cannot be of more assistance on this occasion.

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    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. As my colleague has explained unfortunately this antibody is yet to be tested using mouse and human tissue. However, I would predict its reactivity given 100% sequence similarity. I would like to suggest that you apply the antibody using an overnight incubation at 4oC using a reduced dilution e.g. 1:100. Should this not help, please do not hesitate to get in touch with me again.

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    Answer

    Thank you for the protocol details that you have provided and I'm sorry to hear that you're experiencing difficulty with ab4138. I noticed that you are using human and mouse samples. Ab4138 has been tested for cross-reactivity with rat PKC beta 2 and although it is predicted to cross-react with other mammals dues to sequence homology, it hasn't been tested yet and we can't guarantee results. I checked with the originator of this antibody and the antibody was tested in Western blotting using rat brain, heart, lung and spleen with the highest expression occurring in brain and spleen. I would therefore suggest using rat brain or spleen as a positive control. If this does not help, and you have purchased the antibody in the past 90 days, I would be happy to offer you a free of charge replacement or refund.

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    Answer

    Thank you for your enquiry and for your detailed protocol. This antibody recognizes Rat PKC beta 2 and the predicted molecular weight is kDa. We would like to emphasize the fact that this antibody has only been tested on rat samples. Since you are using human, mouse and hamster samples these cell lines may show different pattern on the Western blot. You may also need to consider the fact that ab4138 is a polyclonal antibody, therefore it recognizes different epitopes. We would strongly suggest running rat samples as positive control along with the cultured cell lines. You may also need to test the secondary antibody to see if there are any non-specific bands due to the secondary antibody. Alternatively, you can decrease the concentration of the primary antibody and apply more stringent washes. Should you still have any problem, please do not hesitate to contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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