Key features and details
- Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PKC delta (phospho S299) antibody [EPNCI119]
See all PKC delta primary antibodies
DescriptionRabbit monoclonal [EPNCI119] to PKC delta (phospho S299)
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide corresponding to residues surrounding Serine 299 of Human PKC delta (Q05655).
- MCF-7 cell lysate treated with Caylculin A; Human transitional cell carcinoma tissue.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
The auto-phosphorylation of PKC delta at pS299 targeted by ab133456 was identified in the following paper: FEBS Lett. 2007 Jul 24;581(18):3377-81. Novel phosphorylation site markers of protein kinase C delta activation. Durgan J1, Michael N, Totty N, Parker PJ.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
modificationsPhosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
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Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma tissue labelling PKC delta phospho S299 with ab133456 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-PKC delta (phospho S299) antibody [EPNCI119] (ab133456) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : MCF-7 cell lysate treated with Caylculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
ab133456 has been referenced in 4 publications.
- Wang N et al. Selective ERK1/2 agonists isolated from Melia azedarach with potent anti-leukemic activity. BMC Cancer 19:764 (2019). PubMed: 31375085
- Li H & Chen C Quercetin Has Antimetastatic Effects on Gastric Cancer Cells via the Interruption of uPA/uPAR Function by Modulating NF-?b, PKC-d, ERK1/2, and AMPKa. Integr Cancer Ther 17:511-523 (2018). PubMed: 28627240
- Kelsey JS et al. Biological activity of the bryostatin analog Merle 23 on mouse epidermal cells and mouse skin. Mol Carcinog 55:2183-2195 (2016). PubMed: 26859836
- Song X et al. RasGRPs are targets of the anti-cancer agent ingenol-3-angelate. PLoS One 8:e72331 (2013). WB ; Human . PubMed: 23991094