Recombinant Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295)
![Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295)](https://www.abcam.com/ps/products/53/ab53295/Images/ab53295-66092-anti-pkc-delta-phospho-t507-antibody-epr1485y-western-blot.png "Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1485Y] to PKC delta (phospho T507)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC delta (phospho T507) antibody [EPR1485Y]
See all PKC delta primary antibodies -
Description
Rabbit monoclonal [EPR1485Y] to PKC delta (phospho T507) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PKC delta (phospho T507). The exact sequence is proprietary.
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Positive control
- U937 cell lysate treated with TPA
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1485Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner. -
Post-translational
modificationsPhosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5580 Human
- Omim: 176977 Human
- SwissProt: Q05655 Human
- Unigene: 155342 Human
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Alternative names
- CVID9 antibody
- D14Ertd420e antibody
- Kinase PKC delta antibody
see all
Images
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Western blot - Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295)All lanes : Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295) at 1/500 dilution
Lane 1 : U937 cell lysate
Lane 2 : U937 cell lysate treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa -
![Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295)](https://www.abcam.com/ps/products/53/ab53295/Images/ab53295-2-benefits-of-recombinant-antibodies.png)
Anti-PKC delta (phospho T507) antibody [EPR1485Y] (ab53295)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab53295 has been referenced in 1 publication.
- Litherland GJ et al. Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction. J Biol Chem 285:22414-25 (2010). PubMed: 20463008
