Western blot abreview for Anti-PKC delta (phospho Y311) antibody [EP2609Y]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Loading amount
40 µg
Specification
C57BL/6 Mouse splenocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA 2% Milk in PBST

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG (H&L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
SuperSignal West Femto
Exposure
10 second(s)
Bands
Specific: 77 kDa Non-specific: 80 kDa

Additional data

Additional Notes
Antibody recognized two proteins at 77kDa and approximately 80kDa.

Dr. Tomar Ghansah

Verified customer

Submitted Oct 09 2012

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