Western blot abreview for Anti-PKC delta (phospho Y311) antibody [EP2609Y]

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cervical Adenocarcinoma, Epithelial)
Loading amount
40 µg
Specification
HeLa Cervical Adenocarcinoma, Epithelial
Treatment
100nM Insulin
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG (H&L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
West Pico
Exposure
30 second(s)
Bands
Specific: none kDa Non-specific: 35, 25 kDa

Additional data

Additional Notes
Lane 1: control HeLA cell lysate probed with anti-PKC delta (phospho Y311).
Lane 2: control HeLA cell lysate probed with probed with anti-Beta actin.
Lane 3: 100nm Insulin treated HeLA cell lysate probed with anti-PKC delta (phospho Y311).
Lane 4: 100nm Insulin treated HeLA cell lysate probed with anti-Beta actin.
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked under your experimental conditions. Many thanks for your feedback on this antibody, we hope that this information will be helpful for future customers.

Dr. Tomar Ghansah

Verified customer

Submitted Feb 20 2013

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