Key features and details
- Rabbit polyclonal to PKC epsilon
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-PKC epsilon antibody
See all PKC epsilon primary antibodies
DescriptionRabbit polyclonal to PKC epsilon
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Rabbit
Recombinant fragment (His-tag) corresponding to Human PKC epsilon aa 403-672. (Expressed in E.coli).
LGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDC TMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSR KFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFG MCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMA GQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGC VASQNGEDAIKQHPFFKEID
Database link: Q02156
- WB: Recombinant human PKC epsilon; Mouse and pig brain lysates; HeLa cell lysate. IHC-P: Human liver, kidney, rectum and thyroid cancer tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233292 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 84 kDa.|
FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsPhosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
- Information by UniProt
- KPCE_HUMAN antibody
- MGC125656 antibody
- MGC125657 antibody
Paraffin-embedded human liver tissue stained for PKC epsilon using ab233292 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for PKC epsilon using ab233292 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human rectum tissue stained for PKC epsilon using ab233292 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human thyroid cancer tissue stained for PKC epsilon using ab233292 at 20μg/ml in immunohistochemical analysis. DAB staining.
Anti-PKC epsilon antibody (ab233292) at 3 µg/ml + Recombinant human PKC epsilon protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa
All lanes : Anti-PKC epsilon antibody (ab233292) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Pig brain lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233292 has not yet been referenced specifically in any publications.