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  1. Link

    pkc-epsilon-peptide-ab204875.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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PKC epsilon peptide (ab204875)

  • Datasheet
  • SDS
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Abpromise

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Key features and details

  • Suitable for: HPLC, Functional Studies

Description

  • Product name

    PKC epsilon peptide
    See all PKC epsilon proteins and peptides
  • Animal free

    No
  • Nature

    Synthetic
    • Sequence

      ERMRPRKRQGSVRRRV

Associated products

  • Recombinant Protein

    • Recombinant human KDS protein (ab125551)
    • Recombinant human MST3 protein (ab51413)
    • Recombinant human PKC theta/PRKCQ protein (ab56641)
    • Recombinant human PKC beta 1 protein (ab60840)
    • Recombinant human PKC gamma protein (ab60842)
    • Recombinant human PKC epsilon protein (ab60847)
    • Recombinant human PKC eta protein (ab60849)
    • Recombinant human STK23 protein (ab84801)

Specifications

Our Abpromise guarantee covers the use of ab204875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    Functional Studies

  • Form

    Lyophilized
  • Additional notes

    ab204875 (PKC epsilon peptide) can be utilized as a substrate for the following active protein kinases:

     

    ab60840 (Active human PKC beta 1 full length protein)
    ab60847 (Active human PKC epsilon full length protein)
    ab60842 (Active human PKC gamma full length protein)
    ab60849 (Active human PKC eta full length protein)
    ab56641 (Active human PKC theta full length protein)
    ab84801 (Active human STK23 full length protein)
    ab51413 (Active human MST3 protein fragment)
    ab125551 (Active human JIK protein fragment)

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

  • Reconstitution
    Dilute peptide in distilled water to a final concentration of 1 mg/ml. For optimal storage, aliquot diluted product into smaller quantities and store at recommended temperature.

General Info

  • Alternative names

    • KPCE_HUMAN
    • MGC125656
    • MGC125657
    • nPKC epsilon
    • nPKC-epsilon
    • PKCE
    • Pkcea
    • PRKCE
    • Protein kinase C epsilon
    • Protein kinase C epsilon type
    see all
  • Function

    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Target information above from: UniProt accession Q02156 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab204875? Please let us know so that we can cite the reference in this datasheet.

ab204875 has not yet been referenced specifically in any publications.

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