Anti-PKC epsilon (phospho S729) antibody (ab63387)
Key features and details
- Rabbit polyclonal to PKC epsilon (phospho S729)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PKC epsilon (phospho S729) antibody
See all PKC epsilon primary antibodies -
Description
Rabbit polyclonal to PKC epsilon (phospho S729) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human PKC epsilon aa 650-750 (phospho S729). Synthetic phosphopeptide from around the phosphorylation site of serine 729 (GFSPYF) of human PKC epsilon
Database link: Q02156 -
Positive control
- WB: HeLa cells treated with PMA (125ng/ml, 30mins).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
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Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsPhosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. - Information by UniProt
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Database links
- Entrez Gene: 5581 Human
- Entrez Gene: 18754 Mouse
- Omim: 176975 Human
- SwissProt: Q02156 Human
- SwissProt: P16054 Mouse
- Unigene: 580351 Human
- Unigene: 24614 Mouse
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Alternative names
- KPCE_HUMAN antibody
- MGC125656 antibody
- MGC125657 antibody
see all
Images
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All lanes : Anti-PKC epsilon (phospho S729) antibody (ab63387) at 1/500 dilution
Lane 1 : HeLa cells treated with PMA (125ng/ml, 30mins) at 30 µg
Lane 2 : Jurkat cells treated eith PMA (125ng/ml, 30mins) at 30 µg
Lane 3 : NIH/3T3 cells treated with PMA (125ng/ml, 30mins) at 30 µg
Lane 4 : NIH/3T3 cells treated with syntesized peptide (125ng/ml, 30mins) at 10 µg
Predicted band size: 84 kDa
Observed band size: 84 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab63387 has been referenced in 24 publications.
- Pang S et al. Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in Tspan7 knockout rats. Life Sci Alliance 6:N/A (2023). PubMed: 36625203
- Ranieri D et al. The FGFR2c/PKCε Axis Controls MCL-1-Mediated Invasion in Pancreatic Ductal Adenocarcinoma Cells: Perspectives for Innovative Target Therapies. Biomedicines 10:N/A (2022). PubMed: 35884957
- Liu F et al. HINT1 Is Involved in the Chronic Mild Stress Elicited Oxidative Stress and Apoptosis Through the PKC e/ALDH-2/4HNE Pathway in Prefrontal Cortex of Rats. Front Behav Neurosci 15:690344 (2021). PubMed: 34177485
- Yoon S et al. Endoplasmic Reticulum Stress Induces CAP2 Expression Promoting Epithelial-Mesenchymal Transition in Liver Cancer Cells. Mol Cells 44:569-579 (2021). PubMed: 34294609
- Jin M et al. Prediction and verification of the AD-FTLD common pathomechanism based on dynamic molecular network analysis. Commun Biol 4:961 (2021). PubMed: 34385591