Anti-PKC eta antibody (ab167482)

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Western blot - Anti-PKC eta antibody (ab167482)

    Key features and details

    • Mouse polyclonal to PKC eta
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-PKC eta antibody
      See all PKC eta primary antibodies

    • Description

      Mouse polyclonal to PKC eta

    • Host species

      Mouse

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Full length Human PKC eta (amino acids 1-683; NP_006246.2).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • PKC eta transfected 293T cell lysate.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab167482 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Horse
    Cow
    Dog
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.

    Target

    • Function

      This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

    • Tissue specificity

      Most abundant in lung, less in heart and skin.

    • Involvement in disease

      Defects in PRKCH may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.

    • Domain

      The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • KPCL_HUMAN antibody
      • MGC 5363 antibody
      • MGC26269 antibody
      • MGC5363 antibody
      • nPKC eta antibody
      • nPKC-eta antibody
      • PKC h antibody
      • PKC L antibody
      • PKC-L antibody
      • PKCh antibody
      • PKCL antibody
      • Prkch antibody
      • PRKCL antibody
      • Protein kinase C eta antibody
      • Protein kinase C eta type antibody
      see all

    Images

    • Western blot - Anti-PKC eta antibody (ab167482)
      Western blot - Anti-PKC eta antibody (ab167482)
      All lanes : Anti-PKC eta antibody (ab167482) at 1 µg/ml

      Lane 1 : PKC eta transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Predicted band size: 78 kDa

    References (0)

    Publishing research using ab167482? Please let us know so that we can cite the reference in this datasheet.

    ab167482 has not yet been referenced specifically in any publications.

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