Recombinant Anti-PKC eta antibody [EPR18513] (ab179524)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18513] to PKC eta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC eta antibody [EPR18513]
See all PKC eta primary antibodies -
Description
Rabbit monoclonal [EPR18513] to PKC eta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Active human PKC eta full length protein; Human fetal brain, fetal heart, fetal skin and fetal kidney lysates; MCF7 and PC-3 whole cell lysates; Mouse skin, lung and brain lysates; Rat skin and brain lysates. IP: Mouse lung whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18513 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 78, 40 kDa (predicted molecular weight: 78 kDa).
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IP |
1/50.
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Notes |
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WB
1/2000. Detects a band of approximately 78, 40 kDa (predicted molecular weight: 78 kDa). |
IP
1/50. |
Target
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Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Most abundant in lung, less in heart and skin. -
Involvement in disease
Defects in PRKCH may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. - Information by UniProt
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Database links
- Entrez Gene: 5583 Human
- Entrez Gene: 18755 Mouse
- Entrez Gene: 81749 Rat
- Omim: 605437 Human
- SwissProt: P24723 Human
- SwissProt: P23298 Mouse
- SwissProt: Q64617 Rat
- Unigene: 333907 Human
see all -
Alternative names
- KPCL_HUMAN antibody
- MGC 5363 antibody
- MGC26269 antibody
see all
Images
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All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution
Lane 1 : Active human PKC alpha full length protein
Lane 2 : Active human PKC beta 1 full length protein
Lane 3 : Active human PKC beta 2 full length protein
Lane 4 : Active human PKC gamma full length protein
Lane 5 : Active human PKC delta full length protein
Lane 6 : Active human PKC eta full length protein
Lane 7 : Active human PKC epsilon full length protein
Lane 8 : Active human PKC theta full length protein
Lane 9 : Active human PKC mu full length protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 78 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein(ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.
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PKC eta was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab179524 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179524 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Mouse lung whole cell lysate 10ug (Input).
Lane 2: ab179524 IP in Mouse lung whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179524 in Mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 45,78 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
(Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498) -
All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 45,78 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
(Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC. 1995. J. Biol. Chem. 270, 28495-28498) -
All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution
Lane 1 : Human fetal skin lysate
Lane 2 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 45,78 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
(Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498) -
All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution
Lane 1 : Rat skin lysate
Lane 2 : Mouse skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 45,78 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
(Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498) -
All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 45,78 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
(Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab179524 has been referenced in 5 publications.
- van Senten JR et al. Use of CRISPR/Cas9-edited HEK293 cells reveals that both conventional and novel protein kinase C isozymes are involved in mGlu5a receptor internalization. J Biol Chem 298:102466 (2022). PubMed: 36087841
- Perveen K et al. Characterization of the Transient Deficiency of PKC Isozyme Levels in Immature Cord Blood T Cells and Its Connection to Anti-Allergic Cytokine Profiles of the Matured Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34884454
- Zeng H et al. Activated PKB/GSK-3ß synergizes with PKC-d signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I. Acta Pharm Sin B 11:71-88 (2021). PubMed: 33532181
- Pohjolainen L et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253
- Liu HY et al. Protein Kinase C-? Deficiency Does Not Impair Antiviral Immunity and CD8+ T Cell Activation. J Immunol 204:2439-2446 (2020). PubMed: 32198145