Overview

  • Product name
    Anti-PKC eta antibody [EPR18513]
    See all PKC eta primary antibodies
  • Description
    Rabbit monoclonal [EPR18513] to PKC eta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse PKC eta aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: P23298

  • Positive control
    • WB: Active human PKC eta full length protein; Human fetal brain, fetal heart, fetal skin and fetal kidney lysates; MCF7 and PC-3 whole cell lysates; Mouse skin, lung and brain lysates; Rat skin and brain lysates. IP: Mouse lung whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR18513
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 78, 40 kDa (predicted molecular weight: 78 kDa).
IP 1/50.

Target

  • Function
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity
    Most abundant in lung, less in heart and skin.
  • Involvement in disease
    Defects in PRKCH may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCL_HUMAN antibody
    • MGC 5363 antibody
    • MGC26269 antibody
    • MGC5363 antibody
    • nPKC eta antibody
    • nPKC-eta antibody
    • PKC h antibody
    • PKC L antibody
    • PKC-L antibody
    • PKCh antibody
    • PKCL antibody
    • Prkch antibody
    • PRKCL antibody
    • Protein kinase C eta antibody
    • Protein kinase C eta type antibody
    see all

Images

  • All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC eta full length protein
    Lane 7 : Active human PKC epsilon full length protein
    Lane 8 : Active human PKC theta full length protein
    Lane 9 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein(ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • PKC eta was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab179524 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179524 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse lung whole cell lysate 10ug (Input).

    Lane 2: ab179524 IP in Mouse lung whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179524 in Mouse lung whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

  • All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC. 1995. J. Biol. Chem. 270, 28495-28498)

  • All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

     

  • All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1 : Rat skin lysate
    Lane 2 : Mouse skin lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

     

  • All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 40,78 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

     

References

ab179524 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

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Application
Flow Cytometry
Sample
Mouse Cell (T cells)
Permeabilization
Yes - Perm Buffer (ebiosciences)
Specification
T cells
Fixation
Fixation buffer (BD Cytofix)

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Verified customer

Submitted Sep 15 2017

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