Overview

  • Product name

  • Description

    Rabbit polyclonal to PKC gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mammals
  • Immunogen

    Synthetic peptide:

    FEACNYPLELYEVSRYGPSSS

    , corresponding to amino acids 302-322 of Human PKC gamma.

Properties

Applications

Our Abpromise guarantee covers the use of ab4144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
IHC-P
IHC-Fr
WB
  • Application notes
    ELISA: assay dependent.
    Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
    Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

    It has also been reported to be useful for immunoprecipitation of PKC gamma.
  • Target

    • Function

      This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity

      Expressed in Purkinje cells of the cerebellar cortex.
    • Involvement in disease

      Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Images

    References

    ab4144 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080 Cell line)
    Specification
    HT1080 Cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100

    Abcam user community

    Verified customer

    Submitted Feb 17 2010

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 Cell line)
    Loading amount
    20000 cells
    Specification
    HT1080 Cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Feb 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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