• Product name
  • Description
    Rabbit polyclonal to PKC gamma
  • Host species
  • Tested applications
    Suitable for: ELISA, IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Drosophila melanogaster
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 684-697 of Rat PKC gamma.

  • Positive control
    • recombinant protein



Our Abpromise guarantee covers the use of ab4145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/250.
WB 1/250. Predicted molecular weight: 84 kDa. using HRP or AP. With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
ICC Use at an assay dependent concentration.


  • Function
    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity
    Expressed in Purkinje cells of the cerebellar cortex.
  • Involvement in disease
    Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCG_HUMAN antibody
    • MGC57564 antibody
    • OTTHUMP00000067291 antibody
    • PKC-gamma antibody
    • PKCC antibody
    • PKCG antibody
    • PRKCG antibody
    • Protein kinase C gamma antibody
    • Protein kinase C gamma polypeptide antibody
    • Protein kinase C gamma type antibody
    • Protein kinase C, gamma antibody
    • SCA 14 antibody
    • SCA14 antibody
    see all


This product has been referenced in:
  • Guo W  et al. Further observations on the behavioral and neural effects of bone marrow stromal cells in rodent pain models. Mol Pain 12:N/A (2016). WB . Read more (PubMed: 27329776) »
  • Rodríguez-Muñoz M  et al. NO-released zinc supports the simultaneous binding of Raf-1 and PKC? cysteine-rich domains to HINT1 protein at the mu-opioid receptor. Antioxid Redox Signal 14:2413-25 (2011). WB ; Mouse . Read more (PubMed: 21235400) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your technical support and interest.
I would suggest applying longer incubation time (overnight instead of 1 hr). Customer may need to use higher final concentration of this primary antibody and longer permeabilization would be also ...

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Thank you for confirming these details. The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the laboratory and your efforts to check the s...

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Thank you for contacting us.

I am sorry to hear this customer has been experiencing problems with one of our PKC gamma antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all custom...

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Thank you for your e-mail. It is good to know that the customer uses a good lysis buffer, secondary antibody and detection system. However, the form you have sent us states the customers used NRK,NR8383, SMC and HSC-T6 cell lines, which are not neuro...

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I can confirm that we recommend a positive control of recombinant protein or as PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons to try brain or spinal cord lysate, my apologies for the delay in c...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. Although ab4145 is predicted to react with mouse, it has not yet been tested so we are unable to guarantee results. The antibody is guaranteed to ...

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Thank you for your patience and for the details that you have provided. What was the lot number (located on the vial) that you received? What was the Abcam order number or purchase order number that was used? We haven't had any other complaints but I w...

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