Western blot abreview for Anti-PKC gamma antibody

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Guinea pig Tissue lysate - other (Cellebreum)
Specification
Cellebreum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Calbiochem goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 78 kDa Non-specific: 35, 45, 68 kDa
Exposure
1 minute(s) and 8 second(s)
Positive control
Mous brain lysate, Rat lysate

Ms. Stephanie Allenfort

Verified customer

Submitted May 23 2014

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