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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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RecombinantRabMAb

Recombinant Anti-PKC gamma antibody [EPR2750] (ab108961)

  • Datasheet
  • SDS
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Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961)
  • Anti-PKC gamma antibody [EPR2750] (ab108961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2750] to PKC gamma
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-PKC gamma antibody [EPR2750]
    See all PKC gamma primary antibodies
  • Description

    Rabbit monoclonal [EPR2750] to PKC gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and Human fetal cerebellum cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2750
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Associated products

  • Alternative Versions

    • Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human PKC gamma protein (ab60842)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity

      Expressed in Purkinje cells of the cerebellar cortex.
    • Involvement in disease

      Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Target information above from: UniProt accession P05129 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5582 Human
      • Entrez Gene: 18752 Mouse
      • Entrez Gene: 24681 Rat
      • Omim: 176980 Human
      • SwissProt: P05129 Human
      • SwissProt: P63318 Mouse
      • SwissProt: P63319 Rat
      • Unigene: 631564 Human
      • Unigene: 7980 Mouse
      • Unigene: 9747 Rat
      see all
    • Alternative names

      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Images

    • Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961)
      Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961)
      All lanes : Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/1000 dilution

      Lane 1 : Human fetal brain cell lysate
      Lane 2 : Human fetal cerebellum cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 78 kDa

    • Anti-PKC gamma antibody [EPR2750] (ab108961)
      Anti-PKC gamma antibody [EPR2750] (ab108961)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab108961? Please let us know so that we can cite the reference in this datasheet.

    ab108961 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-PKC gamma antibody [EPR2750]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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