Recombinant Anti-PKC gamma antibody [EPR2750] (ab108961)
![Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961)](https://www.abcam.com/ps/products/108/ab108961/Images/ab108961-121453-anti-pkc-gamma-antibody-epr2750-western-blot.jpg "Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2750] to PKC gamma
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PKC gamma antibody [EPR2750]
See all PKC gamma primary antibodies -
Description
Rabbit monoclonal [EPR2750] to PKC gamma -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and Human fetal cerebellum cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2750 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa). |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Expressed in Purkinje cells of the cerebellar cortex. -
Involvement in disease
Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA). -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 5582 Human
- Entrez Gene: 18752 Mouse
- Entrez Gene: 24681 Rat
- Omim: 176980 Human
- SwissProt: P05129 Human
- SwissProt: P63318 Mouse
- SwissProt: P63319 Rat
- Unigene: 631564 Human
see all -
Alternative names
- KPCG_HUMAN antibody
- MGC57564 antibody
- OTTHUMP00000067291 antibody
see all
Images
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Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961)All lanes : Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/1000 dilution
Lane 1 : Human fetal brain cell lysate
Lane 2 : Human fetal cerebellum cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa -
![Anti-PKC gamma antibody [EPR2750] (ab108961)](https://www.abcam.com/ps/products/108/ab108961/Images/ab108961-2-benefits-of-recombinant-antibodies.png)
Anti-PKC gamma antibody [EPR2750] (ab108961)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108961 has not yet been referenced specifically in any publications.