• Product name
  • Description
    Rabbit polyclonal to PKC iota
  • Host species
  • Specificity
    Please note, based on blast results, the immunogen of ab154681 PKC iota (Uniprot# P41743) shares 70% identity with human PKC zeta protein (Uniprot# Q05513). Unfortunately, potential cross-reactivity has not been tested.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human PKC iota aa 380-596 (C terminal).

  • Positive control
    • 293T, A431, Jurkat and Raji cell lysates; HeLa cells.
  • General notes

    Please note, based on blast results, the immunogen of ab154681 PKC iota (Uniprot# P41743) shares 70% identity with human PKC zeta protein (Uniprot# Q05513). Unfortunately, potential cross-reactivity has not been tested.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab154681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.


  • Function
    Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs).
  • Tissue specificity
    Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain
    The OPR domain mediates interaction with SQSTM1.
    The C1 domain does not bind diacylglycerol (DAG).
  • Post-translational
    On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
  • Cellular localization
    Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A.
  • Information by UniProt
  • Database links
  • Alternative names
    • aPKC lambda/iota antibody
    • aPKC-lambda/iota antibody
    • Atypical protein kinase C lambda/iota antibody
    • Atypical protein kinase C-lambda/iota antibody
    • DXS1179E antibody
    • KPCI_HUMAN antibody
    • MGC26534 antibody
    • nPKC iota antibody
    • nPKC-iota antibody
    • nPKCiota antibody
    • PKC lambda antibody
    • PKC lambda/iota antibody
    • PKCI antibody
    • PKCiota antibody
    • PKClambda antibody
    • PRKC iota antibody
    • PRKC lambda/iota antibody
    • PRKC-lambda/iota antibody
    • PRKCI antibody
    • Protein kinase C iota antibody
    • Protein kinase C iota type antibody
    see all


  • Anti-PKC iota antibody (ab154681) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 68 kDa

    7.5% SDS PAGE
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling PKC iota with ab154681 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.


ab154681 has not yet been referenced specifically in any publications.

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