Product nameAnti-PKC mu/PKD antibody
See all PKC mu/PKD primary antibodies
DescriptionRabbit polyclonal to PKC mu/PKD
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PKC mu/PKD aa 736-740 conjugated to keyhole limpet haemocyanin.
Database link: NP_002733.2
- Jurkat cytoplasmic extract lysate (ab14842) can be used as a positive control in WB. HeLa cells; HeLa, 293, THP1 and JK cell extracts.
This product was previously labelled as PKC mu
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131460 was purified by affinity chromatography using epitope specific peptide.
Our Abpromise guarantee covers the use of ab131460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa).|
|ICC/IF||1/100 - 1/200.|
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
All lanes : Anti-PKC mu/PKD antibody (ab131460) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : 293 cell extract
Lane 3 : THP1 cell extract
Lane 4 : Jurkat cell extract
Predicted band size: 102 kDa
Immunoflourescent analysis of methanol-fixed HeLa cells labelling PKC mu/PKD with ab131460 at 1/100 dilution.
ab131460 has not yet been referenced specifically in any publications.