Western blot abreview for Anti-PKC mu/PKD antibody

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Astrocytes)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Treatment
Control, Insulin 10nM, IGF-1nM or both hormones 10nM for 15 minutes
Specification
Astrocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TTBS
Concentration
1 µg/ml

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Pierce ECL western blotting susbtrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 90, aprox 85 kDa

Additional data

Additional Notes
A western blot againt phopho PKC mu (ab74105) is showed in the upper panel of the image

Mr. Edwin Hernandez

Verified customer

Submitted May 30 2014

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