Recombinant Anti-PKC mu/PKD antibody [EP1493Y] - BSA and Azide free (ab217484)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1493Y] to PKC mu/PKD - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC mu/PKD antibody [EP1493Y] - BSA and Azide free
See all PKC mu/PKD primary antibodies -
Description
Rabbit monoclonal [EP1493Y] to PKC mu/PKD - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 100% homology with PKD2 and PKD3. Cross-reactivity with these proteins have not been confirmed experimentally.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cell lysate.
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General notes
ab217484 is the carrier-free version of ab51246.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1493Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 105 kDa (predicted molecular weight: 102 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 105 kDa (predicted molecular weight: 102 kDa). |
Target
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Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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Database links
- Entrez Gene: 5587 Human
- Entrez Gene: 18760 Mouse
- Entrez Gene: 85421 Rat
- Omim: 605435 Human
- SwissProt: Q15139 Human
- SwissProt: Q62101 Mouse
- SwissProt: Q9WTQ1 Rat
- Unigene: 508999 Human
see all -
Alternative names
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
Images
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All lanes : Anti-PKC mu/PKD antibody [EP1493Y] (ab51246) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with PMA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?This data was developed using ab51246, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab217484 has not yet been referenced specifically in any publications.