Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1493-62] to PKC mu/PKD (phospho S738 + S742)
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-PKC mu/PKD (phospho S738 + S742) antibody [EP1493-62]
See all PKC mu/PKD primary antibodies
DescriptionRabbit monoclonal [EP1493-62] to PKC mu/PKD (phospho S738 + S742)
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PKC mu/PKD.
- HeLa cell lysate treated with TPA.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PKC mu
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab75878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 102 kDa.|
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
All lanes : Anti-PKC mu/PKD (phospho S738 + S742) antibody [EP1493-62] (ab75878) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
ab75878 has not yet been referenced specifically in any publications.