Recombinant Anti-PKC mu/PKD (phospho S738 + S742) antibody [EP1493-62] (ab75878)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1493-62] to PKC mu/PKD (phospho S738 + S742)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC mu/PKD (phospho S738 + S742) antibody [EP1493-62]
See all PKC mu/PKD primary antibodies -
Description
Rabbit monoclonal [EP1493-62] to PKC mu/PKD (phospho S738 + S742) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cell lysate treated with TPA.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1493-62 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/500. Predicted molecular weight: 102 kDa.
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Notes |
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WB
1/100 - 1/500. Predicted molecular weight: 102 kDa. |
Target
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Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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Database links
- Entrez Gene: 5587 Human
- Omim: 605435 Human
- SwissProt: Q15139 Human
- Unigene: 508999 Human
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Alternative names
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
Images
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All lanes : Anti-PKC mu/PKD (phospho S738 + S742) antibody [EP1493-62] (ab75878) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab75878 has not yet been referenced specifically in any publications.