Product nameAnti-PKC mu/PKD (phospho S910) antibody
See all PKC mu/PKD primary antibodies
DescriptionRabbit polyclonal to PKC mu/PKD (phospho S910)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- 293 cell extract treated with EGF; HUVEC cell extract treated with PMA; HeLa cells
This product was previously labelled as PKC mu
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131267 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa).|
|ICC/IF||1/100 - 1/200.|
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
All lanes : Anti-PKC mu/PKD (phospho S910) antibody (ab131267) at 1/500 dilution
Lane 1 : 293 cell extract treated with EGF
Lane 2 : 293 cell extract untreated
Lane 3 : HUVEC cell extract treated with PMA
Lane 4 : HUVEC cell extract untreated
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of methanol-fixed HeLa cells labelling PKC mu/PKD (phospho S910) with ab131267 at 1/100 dilution.
ab131267 has not yet been referenced specifically in any publications.