• Product name

    Anti-PKC mu/PKD (phospho S916) antibody [EP1494Y]
    See all PKC mu/PKD primary antibodies
  • Description

    Rabbit monoclonal [EP1494Y] to PKC mu/PKD (phospho S916)
  • Host species

  • Specificity

    This antibody detects PKC mu/PKD phosphorylated on Serine 916.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PKC mu/PKD aa 900-1000.

  • Positive control

    • WB: lysate from HeLa cells treated with TPA. IHC: Human placenta tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as PKC mu


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.



Our Abpromise guarantee covers the use of ab81218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Predicted molecular weight: 102 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/60.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Cellular localization

      Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • Database links

    • Alternative names

      • KPCD1_HUMAN antibody
      • nPKC D1 antibody
      • nPKC mu antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • nPKCD1 antibody
      • nPKCmu antibody
      • PKC antibody
      • PKC MU antibody
      • PKCM antibody
      • PKCmu antibody
      • PKD 1 antibody
      • PKD antibody
      • PKD1 antibody
      • PRKCM antibody
      • PRKD 1 antibody
      • Prkd1 antibody
      • Protein kinase C mu antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Protein kinase D1 antibody
      • Serine/threonine protein kinase D1 antibody
      • Serine/threonine-protein kinase D1 antibody
      see all


    • Serum starved HeLa cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of phorbol 12-myristate 13-acetate (PMA) (ab120297) in DMSO. Increased expression of PKC mu/PKD (phospho S916) (ab81218) correlates with an increase in phorbol 12-myristate 13-acetate (PMA) concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab81218 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • All lanes : Anti-PKC mu/PKD (phospho S916) antibody [EP1494Y] (ab81218) at 1/100000 dilution

      Lane 1 : lysate from untreated HeLa cells
      Lane 2 : lyaste from HeLa cells treated with TPA

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 102 kDa

    • Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 ab81218.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.


    This product has been referenced in:

    • Harikumar KB  et al. A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo. Mol Cancer Ther 9:1136-46 (2010). Read more (PubMed: 20442301) »
    See 1 Publication for this product

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