Overview

  • Product name

    Anti-PKC mu/PKD (phospho Y463) antibody
    See all PKC mu/PKD primary antibodies
  • Description

    Rabbit polyclonal to PKC mu/PKD (phospho Y463)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of PKC µ/PKD only when phosphorylated at tyrosine 463 (corresponding to mouse tyrosine 469 or rat tyrosine 200).

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PKC mu/PKD.

  • Positive control

    • Brain tissue
  • General notes

     This product was previously labelled as PKC mu

     

Properties

Applications

Our Abpromise guarantee covers the use of ab59415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • Function

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCD1_HUMAN antibody
    • nPKC D1 antibody
    • nPKC mu antibody
    • nPKC-D1 antibody
    • nPKC-mu antibody
    • nPKCD1 antibody
    • nPKCmu antibody
    • PKC antibody
    • PKC MU antibody
    • PKCM antibody
    • PKCmu antibody
    • PKD 1 antibody
    • PKD antibody
    • PKD1 antibody
    • PRKCM antibody
    • PRKD 1 antibody
    • Prkd1 antibody
    • Protein kinase C mu antibody
    • Protein kinase C mu type antibody
    • Protein kinase D antibody
    • Protein kinase D1 antibody
    • Serine/threonine protein kinase D1 antibody
    • Serine/threonine-protein kinase D1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59415 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

References

This product has been referenced in:

  • Clift D  et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). Read more (PubMed: 29153837) »
  • Zheng H  et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 286:35733-41 (2011). WB ; Human . Read more (PubMed: 21865166) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - other (HeLa cells)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
35 µg
Treatment
3000 IU/ml interferon alpha for 10 min
Specification
HeLa cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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