Key features and details
- Rabbit polyclonal to PKC mu/PKD (phospho Y463)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PKC mu/PKD (phospho Y463) antibody
See all PKC mu/PKD primary antibodies
DescriptionRabbit polyclonal to PKC mu/PKD (phospho Y463)
Detects endogenous levels of PKC µ/PKD only when phosphorylated at tyrosine 463 (corresponding to mouse tyrosine 469 or rat tyrosine 200).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PKC mu/PKD.
This product was previously labelled as PKC mu
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab59415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
All lanes : Anti-PKC mu/PKD (phospho Y463) antibody (ab59415)
Lane 1 : HepG2 cell lysates
Lane 2 : HepG2 cell lysates with Phospho peptide
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59415 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
ab59415 has been referenced in 3 publications.
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Zheng H et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 286:35733-41 (2011). WB ; Human . PubMed: 21865166
- Arun SN et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . PubMed: 21132013