Product nameAnti-PKC theta antibody
See all PKC theta primary antibodies
DescriptionRabbit polyclonal to PKC theta
Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mammals
Synthetic peptide corresponding to a distinct C terminal region (Rat). H-S-N-F-S-F-I-N-P-G-M-E-T-L-I-C-S
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab4133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry: 1/250 (alkaline phosphatase or horseradish peroxidase).
Western blot (enhanced chemiluminescence: 1/250 (alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 85 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as
enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for
immunoprecipitation of PKC theta.
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
ab4133 has not yet been referenced specifically in any publications.