Anti-PKC theta/PRKCQ (phospho T538) antibody (ab63365)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ (phospho T538)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC theta/PRKCQ (phospho T538) antibody
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit polyclonal to PKC theta/PRKCQ (phospho T538) -
Host species
Rabbit -
Specificity
ab63365 detects PKC theta/PRKCQ only when phosphorylated at threonine 538.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human PKC theta/PRKCQ aa 500-600 (phospho T538).
Database link: Q04759 -
Positive control
- Jurkat cell extracts and human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). |
Target
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Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Skeletal muscle, megakaryoblastic cells and platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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Database links
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- Unigene: 498570 Human
- Unigene: 329993 Mouse
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Alternative names
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
Images
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All lanes : Anti-PKC theta/PRKCQ (phospho T538) antibody (ab63365) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : Jurkat cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa -
ab63365 at 1/50 dilution staining PKC theta/PRKCQ in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab63365 has been referenced in 2 publications.
- Boulding T et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). PubMed: 29311580
- McCuaig RD et al. PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation. Front Immunol 6:562 (2015). ICC/IF ; Human . PubMed: 26594212