Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PKC theta/PRKCQ antibody
See all PKC theta/PRKCQ primary antibodies
DescriptionRabbit polyclonal to PKC theta/PRKCQ
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- HeLa cells, PC12 cell extracts, Human lung carcinoma tissue.
This product was previously labelled as PKC theta
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody detects endogenous levels of total PKC theta/PRKCQ protein.
Our Abpromise guarantee covers the use of ab131503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 82 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
Anti-PKC theta/PRKCQ antibody (ab131503) at 1/500 dilution + PC12 cell extract
Predicted band size: 82 kDa
Immunofluorescence analysis of methanol fixed HeLa cells (ab150035) labelled with ab131503 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelled with ab131503 at 1/50 dilution (left) and ab131503 preincubated with blocking peptide (right).
ab131503 has not yet been referenced specifically in any publications.