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  1. Link

    pkc-thetaprkcq-antibody-ab185974.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Anti-PKC theta/PRKCQ antibody (ab185974)

  • Datasheet
Reviews (1) Submit a question References (1)

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Immunoprecipitation - Anti-PKC theta/PRKCQ antibody (ab185974)
  • Western blot - Anti-PKC theta/PRKCQ antibody (ab185974)

Key features and details

  • Rabbit polyclonal to PKC theta/PRKCQ
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human PKC theta/PRKCQ protein (ab56641)

View more associated products

Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit polyclonal to PKC theta/PRKCQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PKC theta/PRKCQ aa 656-706. The exact sequence is proprietary.
    Sequence:

    KSPFD CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG MERLIS


    Database link: NP_006248.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as PKC theta

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human PKC theta/PRKCQ protein (ab56641)
  • Related Products

    • Anti-PKC theta/PRKCQ antibody (ab131503)

Applications

Our Abpromise guarantee covers the use of ab185974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Target information above from: UniProt accession Q04759 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5588 Human
    • Entrez Gene: 18761 Mouse
    • Entrez Gene: 85420 Rat
    • Omim: 600448 Human
    • SwissProt: Q04759 Human
    • SwissProt: Q02111 Mouse
    • SwissProt: Q9WTQ0 Rat
    • Unigene: 498570 Human
    • Unigene: 329993 Mouse
    • Unigene: 225125 Rat
    see all
  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • Immunoprecipitation - Anti-PKC theta/PRKCQ antibody (ab185974)
    Immunoprecipitation - Anti-PKC theta/PRKCQ antibody (ab185974)

    Detection of PKC theta/PRKCQ by Western Blot of Immunoprecipitated jurkat whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185974, for IP at 6 µg/mg lysate and for blotting at 1 µg/ml.

  • Western blot - Anti-PKC theta/PRKCQ antibody (ab185974)
    Western blot - Anti-PKC theta/PRKCQ antibody (ab185974)
    Anti-PKC theta/PRKCQ antibody (ab185974) at 0.1 µg/ml + Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 82 kDa


    Exposure time: 3 minutes

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab185974? Please let us know so that we can cite the reference in this datasheet.

    ab185974 has been referenced in 1 publication.

    • Dybas JM  et al. Integrative proteomics reveals an increase in non-degradative ubiquitylation in activated CD4+ T cells. Nat Immunol 20:747-755 (2019). PubMed: 31061531

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-PKC theta antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (muscle)
    Specification
    muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    colleague

    Verified customer

    Submitted Nov 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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