Anti-PKC theta/PRKCQ antibody (ab185974)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC theta/PRKCQ antibody
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit polyclonal to PKC theta/PRKCQ -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human PKC theta/PRKCQ aa 656-706. The exact sequence is proprietary.
Sequence:KSPFD CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG MERLIS
Database link: NP_006248.1 -
Positive control
- Jurkat whole cell lysate.
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General notes
This product was previously labelled as PKC theta
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab185974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 82 kDa. |
Target
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Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Skeletal muscle, megakaryoblastic cells and platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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Database links
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
see all -
Alternative names
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
Images
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Immunoprecipitation - Anti-PKC theta/PRKCQ antibody (ab185974)
Detection of PKC theta/PRKCQ by Western Blot of Immunoprecipitated jurkat whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185974, for IP at 6 µg/mg lysate and for blotting at 1 µg/ml.
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Western blot - Anti-PKC theta/PRKCQ antibody (ab185974)Anti-PKC theta/PRKCQ antibody (ab185974) at 0.1 µg/ml + Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
References (1)
ab185974 has been referenced in 1 publication.
- Dybas JM et al. Integrative proteomics reveals an increase in non-degradative ubiquitylation in activated CD4+ T cells. Nat Immunol 20:747-755 (2019). PubMed: 31061531
