Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit polyclonal to PKC theta/PRKCQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PKC theta/PRKCQ aa 656-706. The exact sequence is proprietary.
    Sequence:

    KSPFD CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG MERLIS


    Database link: NP_006248.1

  • Positive control

    • Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as PKC theta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab185974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • Detection of PKC theta/PRKCQ by Western Blot of Immunoprecipitated jurkat whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185974, for IP at 6 µg/mg lysate and for blotting at 1 µg/ml.

  • Anti-PKC theta/PRKCQ antibody (ab185974) at 0.1 µg/ml + Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 82 kDa


    Exposure time: 3 minutes

References

ab185974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (muscle)
Specification
muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

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Verified customer

Submitted Nov 19 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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