Overview

  • Product name
    Anti-PKC theta/PRKCQ antibody
    See all PKC theta/PRKCQ primary antibodies
  • Description
    Rabbit polyclonal to PKC theta/PRKCQ
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mammals
  • Immunogen

    Synthetic peptide corresponding to Rat PKC theta/PRKCQ (C terminal).
    Sequence:

    H-S-N-F-S-F-I-N-P-G-M-E-T-L-I-C-S

  • General notes

     This product was previously labelled as PKC theta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab4133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
IHC-Fr
WB
  • Application notes
    ELISA: assay dependent.
    Immunohistochemistry: 1/250 (alkaline phosphatase or horseradish peroxidase).
    Western blot (enhanced chemiluminescence: 1/250 (alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 85 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as
    enhanced chemiluminescence or ELISA, the antibody may be diluted further.

    It has also been reported to be useful for
    immunoprecipitation of PKC theta.
  • Target

    • Function
      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity
      Skeletal muscle, megakaryoblastic cells and platelets.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Domain
      The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Post-translational
      modifications
      Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCT_HUMAN antibody
      • MGC126514 antibody
      • MGC141919 antibody
      • nPKC theta antibody
      • nPKC-theta antibody
      • nPKCtheta antibody
      • OTTHUMP00000043364 antibody
      • OTTHUMP00000043365 antibody
      • PKC 0 antibody
      • PKC0 antibody
      • Prkcq antibody
      • PRKCT antibody
      • Protein kinase C theta antibody
      • Protein kinase C theta type antibody
      • Protein Kinase Ctheta antibody
      see all

    References

    ab4133 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer



    The amino acid sequence is H-S-N-F-S-F-I-N-P-G-M-E-T-L-I-C-S

    Read More

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