Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PKC theta/PRKCQ antibody - C-terminal
See all PKC theta/PRKCQ primary antibodies
DescriptionRabbit polyclonal to PKC theta/PRKCQ - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Cow, Rhesus monkey
Recombinant fragment within Human PKC theta/PRKCQ (C terminal). The exact sequence is proprietary.
Database link: Q04759
- WB: HeLa and HepG2 whole cell extracts. IHC-P: Rat spleen and lymph node tissue.
This product was previously labelled as PKC theta
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 82 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
All lanes : Anti-PKC theta/PRKCQ antibody - C-terminal (ab227155) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 82 kDa
Paraffin embedded rat lymph node tissue stained for PKC theta/PRKCQ with ab227155 (1/500 dilution) in immunohistochemical analysis.
Paraffin embedded rat spleen tissue stained for PKC theta/PRKCQ with ab227155 (1/500 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227155 has not yet been referenced specifically in any publications.