Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody - C-terminal
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit polyclonal to PKC theta/PRKCQ - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PKC theta/PRKCQ (C terminal). The exact sequence is proprietary.
    Database link: Q04759

  • Positive control

    • WB: HeLa and HepG2 whole cell extracts. IHC-P: Rat spleen and lymph node tissue.
  • General notes

     This product was previously labelled as PKC theta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • All lanes : Anti-PKC theta/PRKCQ antibody - C-terminal (ab227155) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 82 kDa



    7.5% SDS-PAGE

  • Paraffin embedded rat lymph node tissue stained for PKC theta/PRKCQ with ab227155 (1/500 dilution) in immunohistochemical analysis.

  • Paraffin embedded rat spleen tissue stained for PKC theta/PRKCQ with ab227155 (1/500 dilution) in immunohistochemical analysis.

References

ab227155 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227155.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up