Recombinant Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-PKC theta/PRKCQ antibody [EPR22277]
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit monoclonal [EPR22277] to PKC theta/PRKCQ -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PKC theta/PRKCQ aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: Q04759 -
Positive control
- WB: Jurkat, MOLT-4 and K562 whole cell lysate. IHC-P: Human tonsil tissue. Human gastrointestinal stromal tumor (GIST). IP: PKC theta/PRKCQ IP in K562 whole cell lysate.
-
General notes
This product was previously labelled as PKC theta
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22277 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Immunohistochemistry reagents
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab230971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 82 kDa. | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | 1/30. |
Target
-
Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Skeletal muscle, megakaryoblastic cells and platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
-
Database links
- Entrez Gene: 5588 Human
- Omim: 600448 Human
- SwissProt: Q04759 Human
- Unigene: 498570 Human
-
Alternative names
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)](https://www.abcam.com/ps/products/230/ab230971/Images/ab230971-4-230971IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human gastrointestinal stromal tumor (PMID: 15289315) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)](https://www.abcam.com/ps/products/230/ab230971/Images/ab230971-3-230971IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in T lymphocytes in tonsil (PMID: 27149922) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)](https://www.abcam.com/ps/products/230/ab230971/Images/ab230971-5-230971IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control: No staining in human leiomyosarcoma (PMID: 15289315).
Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Western blot - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)](https://www.abcam.com/ps/products/230/ab230971/Images/ab230971-2-230971WB1.jpg)
Western blot - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)All lanes : Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 75, 40 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature. The 40kDa band is a cleaved form (PMID: 9252332, PMID: 24924627).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds; Lanes 2-3: 3 minutes.
-
![Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)](https://www.abcam.com/ps/products/230/ab230971/Images/ab230971-1-230971IP1.jpg)
Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)PKC theta/PRKCQ was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab230971 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230971 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: K562 whole cell lysate 10 µg (Input).
Lane 2: ab230971 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230971 in K562 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.
Datasheets and documents
References
ab230971 has not yet been referenced specifically in any publications.
