Recombinant
RabMAb

Recombinant Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit monoclonal [EPR22281] to PKC theta/PRKCQ - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PKC theta/PRKCQ aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04759

  • Positive control

    • IHC-P: Human tonsil and gastrointestinal stromal tumor tissues. Flow cyt: Jurkat and K562 cells.
  • General notes

    ab243853 is the carrier-free version of ab230972 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab243853 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PKC theta

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 75, 40 kDa (predicted molecular weight: 82 kDa).
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity

      Skeletal muscle, megakaryoblastic cells and platelets.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Domain

      The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Post-translational
      modifications

      Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
    • Information by UniProt
    • Database links

    • Alternative names

      • KPCT_HUMAN antibody
      • MGC126514 antibody
      • MGC141919 antibody
      • nPKC theta antibody
      • nPKC-theta antibody
      • nPKCtheta antibody
      • OTTHUMP00000043364 antibody
      • OTTHUMP00000043365 antibody
      • PKC 0 antibody
      • PKC0 antibody
      • Prkcq antibody
      • PRKCT antibody
      • Protein kinase C theta antibody
      • Protein kinase C theta type antibody
      • Protein Kinase Ctheta antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining in T lymphocytes of tonsil (PMID: 27149922) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

    • Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human gastrointestinal stromal tumor (PMID: 15289315) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

    • Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining in human leiomyosarcoma (PMID: 15289315) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling PKC theta/PRKCQ with ab230972 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling PKC theta/PRKCQ with ab230972 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

    References

    ab243853 has not yet been referenced specifically in any publications.

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