• Product name

    Anti-PKC theta/PRKCQ antibody [M217]
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Mouse monoclonal [M217] to PKC theta/PRKCQ
  • Host species

  • Specificity

    ab76197 is not predicted to react with other PKC family members due to low sequence homology.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Mouse PKC theta/PRKCQ (N terminal).

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). ICC/IF: PC-12 cells.
  • General notes

     This product was previously labelled as PKC theta




Our Abpromise guarantee covers the use of ab76197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 82 kDa.
ELISA 1/2000.


  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all


  • Lane 1 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/250 dilution
    Lane 2 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate.
    Lane 2 : Jurkat cell lysate

    Predicted band size: 82 kDa
    Observed band size: 79 kDa
    why is the actual band size different from the predicted?

  • NGF-differentiated PC-12 cells (rat adrenal gland pheochromocytoma cell line) stained for PKC theta/PRKCQ using ab76197 at 1/100 dilution in ICC/IF. Secondary antibody is a Cy3 conjugate.


ab76197 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab76197.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up