Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody [M217]
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Mouse monoclonal [M217] to PKC theta/PRKCQ
  • Host species

    Mouse
  • Specificity

    ab76197 is not predicted to react with other PKC family members due to low sequence homology.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Mouse PKC theta/PRKCQ (N terminal).

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). ICC/IF: PC-12 cells.
  • General notes

     This product was previously labelled as PKC theta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 82 kDa.
ELISA 1/2000.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • Lane 1 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/250 dilution
    Lane 2 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate.
    Lane 2 : Jurkat cell lysate

    Predicted band size: 82 kDa
    Observed band size: 79 kDa
    why is the actual band size different from the predicted?

  • NGF-differentiated PC-12 cells (rat adrenal gland pheochromocytoma cell line) stained for PKC theta/PRKCQ using ab76197 at 1/100 dilution in ICC/IF. Secondary antibody is a Cy3 conjugate.

References

ab76197 has not yet been referenced specifically in any publications.

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