• Product name

    Anti-PKC theta/PRKCQ (phospho S676) antibody
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit polyclonal to PKC theta/PRKCQ (phospho S676)
  • Host species

  • Specificity

    ab131479 detects endogenous levels of PKC theta/PRKCQ only when phosphorylated at serine 676.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PKC theta/PRKCQ conjugated to keyhole limpet haemocyanin.


  • Positive control

    • HeLa cells; PC12 cell extract; Human lung carcinoma tissue.
  • General notes

     This product was previously labelled as PKC theta




Our Abpromise guarantee covers the use of ab131479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.


  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all


  • All lanes : Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479) at 1/500 dilution

    Lane 1 : PC12 cell extract
    Lane 2 : PC12 cell extract treated with PMA

    Predicted band size: 82 kDa

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling PKC theta/PRKCQ (phospho S676) with ab131479 at 1/50 dilution (left) or ab131479 preincubated with blocking peptide (right).

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling PKC theta/PRKCQ (phospho S676) with ab131479 at 1/100 dilution.


This product has been referenced in:

  • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30871020) »
  • Søgaard D  et al. Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity. J Diabetes Res 2016:2372741 (2016). Read more (PubMed: 27777958) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131479.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up