Product nameAnti-PKC theta/PRKCQ (phospho S676) antibody
See all PKC theta/PRKCQ primary antibodies
DescriptionRabbit polyclonal to PKC theta/PRKCQ (phospho S676)
ab131479 detects endogenous levels of PKC theta/PRKCQ only when phosphorylated at serine 676.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- HeLa cells; PC12 cell extract; Human lung carcinoma tissue.
This product was previously labelled as PKC theta
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131479 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
All lanes : Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479) at 1/500 dilution
Lane 1 : PC12 cell extract
Lane 2 : PC12 cell extract treated with PMA
Predicted band size: 82 kDa
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling PKC theta/PRKCQ (phospho S676) with ab131479 at 1/50 dilution (left) or ab131479 preincubated with blocking peptide (right).
Immunofluorescent analysis of methanol-fixed HeLa cells labelling PKC theta/PRKCQ (phospho S676) with ab131479 at 1/100 dilution.
This product has been referenced in:
- Søgaard D et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30871020) »
- Søgaard D et al. Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity. J Diabetes Res 2016:2372741 (2016). Read more (PubMed: 27777958) »