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  1. Link

    pkc-thetaprkcq-phospho-s695-antibody-ab75658.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Anti-PKC theta/PRKCQ (phospho S695) antibody (ab75658)

  • Datasheet
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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S695) antibody (ab75658)

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Primary

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-PKC theta/PRKCQ (phospho S695) antibody
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit polyclonal to PKC theta/PRKCQ (phospho S695)
  • Host species

    Rabbit
  • Specificity

    ab75658 detects endogenous levels of PKC theta/PRKCQ only when phosphorylated at serine 695.

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PKC theta/PRKCQ.

  • Positive control

    • Human lung carcinoma tissue.
  • General notes

     This product was previously labelled as PKC theta

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab75658 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human PKC theta/PRKCQ protein (ab56641)

Applications

Our Abpromise guarantee covers the use of ab75658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Target information above from: UniProt accession Q04759 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5588 Human
    • Entrez Gene: 18761 Mouse
    • Entrez Gene: 85420 Rat
    • Omim: 600448 Human
    • SwissProt: Q04759 Human
    • SwissProt: Q02111 Mouse
    • SwissProt: Q9WTQ0 Rat
    • Unigene: 498570 Human
    • Unigene: 329993 Mouse
    • Unigene: 225125 Rat
    see all
  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S695) antibody (ab75658)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S695) antibody (ab75658)

    ab75658 at 1/50 dilution staining PKC theta/PRKCQ in human lung carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

Protocols

  • Immunohistochemistry protocols

Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Zafar A  et al. Chromatinized protein kinase C-? directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells. Mol Cell Biol 34:2961-80 (2014). Read more (PubMed: 24891615) »
    See 1 Publication for this product

    Publishing research using ab75658? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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