Product nameAnti-PKC theta/PRKCQ (phospho T538) antibody
See all PKC theta/PRKCQ primary antibodies
DescriptionRabbit polyclonal to PKC theta/PRKCQ (phospho T538)
ab63365 detects PKC theta/PRKCQ only when phosphorylated at threonine 538.
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human PKC theta/PRKCQ.
- Jurkat cell extracts and human brain tissue.
This product was previously labelled as PKC theta
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).|
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
All lanes : Anti-PKC theta/PRKCQ (phospho T538) antibody (ab63365) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : Jurkat cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa
ab63365 at 1/50 dilution staining PKC theta/PRKCQ in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
This product has been referenced in:
- Boulding T et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). Read more (PubMed: 29311580) »
- McCuaig RD et al. PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation. Front Immunol 6:562 (2015). ICC/IF ; Human . Read more (PubMed: 26594212) »