Overview

  • Product name

    Anti-PKC theta/PRKCQ (phospho T538) antibody
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit polyclonal to PKC theta/PRKCQ (phospho T538)
  • Host species

    Rabbit
  • Specificity

    ab63365 detects PKC theta/PRKCQ only when phosphorylated at threonine 538.

  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PKC theta/PRKCQ.

  • Positive control

    • Jurkat cell extracts and human brain tissue.
  • General notes

     This product was previously labelled as PKC theta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab63365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • All lanes : Anti-PKC theta/PRKCQ (phospho T538) antibody (ab63365) at 1/500 dilution

    Lane 1 : Jurkat cell extracts
    Lane 2 : Jurkat cell extracts with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 82 kDa
    Observed band size: 82 kDa

  • ab63365 at 1/50 dilution staining PKC theta/PRKCQ in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:

  • Boulding T  et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). Read more (PubMed: 29311580) »
  • McCuaig RD  et al. PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation. Front Immunol 6:562 (2015). ICC/IF ; Human . Read more (PubMed: 26594212) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry.



In this case I would suggest using acapture ELISA assay. Here, the capture antibody will be an antibodyto capture total PKC theta and thedetection antibody will be yourab63365. For the capture antibody you can try https://www.abcam.com/PKC-theta-antibody-ab4133.html, although this has not yetbeen tested as capture antibody.

Then you'll need a secondary antibody, conjugated to an enzyme for example, to detect this detection antibody. One example of a secondary antibody you could use is https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7083.html

For apositive control/standard curve, you canuse our recombinant full length PKC theta protein, https://www.abcam.com/PKC-theta-protein-Tagged-ab56641.html

I hope this is helpful. Please contact me again if you have any further questions.

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