Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [F4H4L1] to PKC theta/PRKCQ (phospho T538)
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1]
See all PKC theta/PRKCQ primary antibodies
DescriptionRabbit monoclonal [F4H4L1] to PKC theta/PRKCQ (phospho T538)
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Jurkat cells and lysates stimulated with PMA; HeLa cells; Human breast carcinoma tissue.
This product was previously labelled as PKC theta
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 82 kDa.|
|IHC-P||Use a concentration of 4 - 6 µg/ml.|
|Flow Cyt||Use a concentration of 8 - 12 µg/ml.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.
0.1 - 0.5 μg/test.
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
All lanes : Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1] (ab203565) at 3 µg/ml
Lane 1 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour
Lane 2 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Immunogen phosphopeptide
Lane 3 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Non-phosphopeptide
Predicted band size: 82 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PKC theta/PRKCQ (phospho T538) with ab203565 at 5 µg/mL followed by DAB staining. Magnification: 20x.
Immunofluorescence analysis of HeLa cells labeling PKC theta/PRKCQ (phospho T538) with ab503565 at 10 μg/mL in the absence of peptides (top panels), presence of phosphopeptide used as immunogen (bottom panels) or non-phosphopeptide (middle panels). Alexa Fluor® 488 goat anti-rabbit used at 1/1000 was used as secondary antibody. Actin was stained with Alexa Fluor® 568 Phalloidin. Hoechst only (left), PKC-θ [pT538] (AF488) signal only (left center), composite image with Phalloidin (right center), and composite image without Hoechst (right).
Flow cytometric analysis of Jurkat cells (incubated with 100 μM PMA for 1 hour prior to being fixed and permeabilized) labeling PKC theta/PRKCQ (phospho T538 with ab203565 at 0.1 μg followed by Alexa Fluor® 488 goat anti-rabbit IgG (grey). Pre-incubation with the immunogenic phosphopeptide decreased the signal (red), whereas incubation with the non-phosphopeptide did not (blue).
ab203565 has not yet been referenced specifically in any publications.