Recombinant

Recombinant Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1] (ab203565)

Overview

  • Product name

    Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1]
    See all PKC theta/PRKCQ primary antibodies
  • Description

    Rabbit monoclonal [F4H4L1] to PKC theta/PRKCQ (phospho T538)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PKC theta/PRKCQ aa 531-539 (phospho T538).
    Sequence:

    LGDAKTNTF


    Database link: Q04759

  • Positive control

    • Jurkat cells and lysates stimulated with PMA; HeLa cells; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as PKC theta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab203565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 82 kDa.
IHC-P Use a concentration of 4 - 6 µg/ml.
Flow Cyt Use a concentration of 8 - 12 µg/ml.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

0.1 - 0.5 μg/test.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Skeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links

  • Alternative names

    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Images

  • All lanes : Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1] (ab203565) at 3 µg/ml

    Lane 1 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour
    Lane 2 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Immunogen phosphopeptide
    Lane 3 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Non-phosphopeptide

    Predicted band size: 82 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PKC theta/PRKCQ (phospho T538) with ab203565 at 5 µg/mL followed by DAB staining. Magnification: 20x.

  • Immunofluorescence analysis of HeLa cells labeling PKC theta/PRKCQ (phospho T538) with ab503565 at 10 μg/mL in the absence of peptides (top panels), presence of phosphopeptide used as immunogen (bottom panels) or non-phosphopeptide (middle panels). Alexa Fluor® 488 goat anti-rabbit used at 1/1000 was used as secondary antibody. Actin was stained with Alexa Fluor® 568 Phalloidin. Hoechst only (left), PKC-θ [pT538] (AF488) signal only (left center), composite image with Phalloidin (right center), and composite image without Hoechst (right).

  • Flow cytometric analysis of Jurkat cells (incubated with 100 μM PMA for 1 hour prior to being fixed and permeabilized) labeling PKC theta/PRKCQ (phospho T538 with ab203565 at 0.1 μg followed by Alexa Fluor® 488 goat anti-rabbit IgG (grey). Pre-incubation with the immunogenic phosphopeptide decreased the signal (red), whereas incubation with the non-phosphopeptide did not (blue).

References

ab203565 has not yet been referenced specifically in any publications.

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